Principles and application of fluorescence microscopy.

@article{Coling1998PrinciplesAA,
  title={Principles and application of fluorescence microscopy.},
  author={D E Coling and Bechara Kachar},
  journal={Current protocols in molecular biology},
  year={1998},
  volume={Chapter 14},
  pages={
          Unit 14.10
        }
}
This overview discusses the principle of fluorescence along with practical discussions of fluorescent molecular probes, filters and filter sets, multiband filters and multi-dye fluorescence, light sources, objective lenses, image resolution and the point-spread function, fluorescence microscopy of living cells, and immunolabeling. 
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