Primer design for large scale sequencing.

  title={Primer design for large scale sequencing.},
  author={Stefan A. Haas and Martin Vingron and Annemarie Poustka and Stefan Wiemann},
  journal={Nucleic acids research},
  volume={26 12},
We have developed PRIDE, a primer design program that automatically designs primers in single contigs or whole sequencing projects to extend the already known sequence and to double strand single-stranded regions. The program is fully integrated into the Staden package (GAP4) and accessible with a graphical user interface. PRIDE uses a fuzzy logic-based system to calculate primer qualities. The computational performance of PRIDE is enhanced by using suffix trees to store the huge amount of data… CONTINUE READING
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  • P Li, K C Kupfer, C J Davies, D Burbee, G A Evans, H R Garner
  • Genomics
  • 1997

Comput. Applicat. Biol. Sci

  • V Proutski, E C Holmes
  • Comput. Applicat. Biol. Sci
  • 1996

Fuzzy Logic, Vols 1–3

  • Fuzzy Logic, Vols 1–3
  • 1995

Nature Genet

  • P A Ioannou, C T Amemiya, +5 authors P J De Jong
  • Nature Genet
  • 1994


  • E Ukkonen
  • 1993

Proc. Natl. Acad. Sci. USA

  • H Shizuya, B Birren, +4 authors M Simon
  • Proc. Natl. Acad. Sci. USA
  • 1992

Programming with GAP4 v.0.99.2, User Manual

  • J Bonfield
  • MRC Laboratory of Molecular Biology Nucleic Acids…
  • 1992

PCR Methods Applicat

  • Hillier, L Green
  • PCR Methods Applicat
  • 1991


  • T Hunkapiller, R J Kaiser, B F Koop, L E Hood
  • Science
  • 1991

Anal. Biochem

  • E C Strauss, J A Kobori, G Siu, L E Hood
  • Anal. Biochem
  • 1986

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