Primer and probe sets for group-specific quantification of the genera Nitrosomonas and Nitrosospira using real-time PCR.

@article{Lim2008PrimerAP,
  title={Primer and probe sets for group-specific quantification of the genera Nitrosomonas and Nitrosospira using real-time PCR.},
  author={Juntaek Lim and Hyojin Do and Seung Gu Shin and Seokhwan Hwang},
  journal={Biotechnology and bioengineering},
  year={2008},
  volume={99 6},
  pages={1374-83}
}
Use of quantitative real-time PCR (QPCR) with TaqMan probes is increasingly popular in various environmental works to detect and quantify a specific microorganism or a group of target microorganism. Although many aspects of conducting a QPCR assay have become very easy to perform, a proper design of oligonucleotide sequences comprising primers and a probe is still considered as one of the most important aspects of a QPCR application. This work was conducted to design group specific primer and… CONTINUE READING
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