Primer Master: a new program for the design and analysis of PCR primers

@article{Proutski1996PrimerMA,
  title={Primer Master: a new program for the design and analysis of PCR primers},
  author={V. Proutski and Edward C. Holmes},
  journal={Computer applications in the biosciences : CABIOS},
  year={1996},
  volume={12 3},
  pages={253-5}
}
The polymerase chain reaction (PCR) is one of the most rapidly expanding methods in molecular biology. Although the range of applications of PCR is very broad, the key points for successful performance remain the careful selection of optimal primers and the proper determination of the temperature conditions of the reaction. The program presented here, 'Primer Master', has been developed to assist in solving these problems for two major types of PCR: the amplification of the appropriate… CONTINUE READING