Prime time for real-time PCR

@article{Bonetta2005PrimeTF,
  title={Prime time for real-time PCR},
  author={Laura P. Bonetta},
  journal={Nature Methods},
  year={2005},
  volume={2},
  pages={305-312}
}
  • L. Bonetta
  • Published 1 April 2005
  • Biology
  • Nature Methods
Real-time PCR is the favored method for measuring gene expression. Researchers benefit from a vast and growing choice of reagents and instruments for their experiments. Laura Bonetta reports. 

Gene expression: one size does not fit all

Although in recent years the field has been flooded with microarray data, a multitude of non–array-based methods for studying the expression of genes are on the market.

Ligation-Enabled Fluorescence-Coding PCR for High-Dimensional Fluorescence-Based Nucleic Acid Detection.

The LiNC PCR technique is demonstrated by implementing a two-color-based assay for detection of 10 ovarian cancer epigenetic biomarkers at analytical sensitivities as low as 60 template molecules with no detectable target cross-talk.

PCR-Stop analysis as a new tool for qPCR assay validation

The benefits of PCR-Stop analysis reveal quantitative and qualitative resolution of both assays, the limits of one of those assays and thus avoiding misinterpretations in qPCR analysis, and data displayed that a well performing assay starts indeed with its average efficiency.

A Fast-and-Robust Profiler for Improving Polymerase Chain Reaction Diagnostics

A profiling method is proposed that permits the non-linear maximization of amplicon resolution by eliminating the necessity for direct error estimation and is expected to have extensive applications in genetics and biotechnology where there is a demand for cheap, expedient, and robust information.

Real-time PCR assay for the discrimination and quantification of wheat and barley strains of Wheat dwarf virus

A rapid method for detection, discrimination and quantification of wheat and barley strains of wheat dwarf virus (WDV) was successfully developed and both TaqMan® and SYBR® Green technologies provided accurate and reliable methods.

Fluorescence Polarization Based Nucleic Acid Testing for Rapid and Cost-Effective Diagnosis of Infectious Disease.

A new nucleic acid detection method was developed for a rapid and cost-effective diagnosis of infectious disease and was used to detect and sub-type Salmonella bacteria with sensitivities down to a single bacterium in less than three hours.

Detection and Serotype-Specific Differentiation of Vesicular Stomatitis Virus Using a Multiplex, Real-Time, Reverse Transcription-Polymerase Chain Reaction Assay

  • K. HoleA. ClavijoLuis A Pineda
  • Medicine, Biology
    Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
  • 2006
The real-time RT-PCR assay was documented to be sensitive and specific for the detection ofVSV-NJ and VSV-IN (1–3) strains from field samples in a single reaction, thereby supporting use of this assay in the differential diagnosis of vesicular virus diseases in cattle.

The Qiagen Investigator® Quantiplex HYres as an alternative kit for DNA quantification.

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Laura Bonetta is a freelance writer based in the Washington, DC area. (lbonetta@nasw.org)

  • Laura Bonetta is a freelance writer based in the Washington, DC area. (lbonetta@nasw.org)