Primary structure of guinea-pig Hageman factor: sequence around the cleavage site differs from the human molecule.

@article{Semba1992PrimarySO,
  title={Primary structure of guinea-pig Hageman factor: sequence around the cleavage site differs from the human molecule.},
  author={Umeko Semba and Takenori Yamamoto and T Kunisada and Y. Shibuya and Sumio Tanase and Takeshi Kambara and Hiroaki Okabe},
  journal={Biochimica et biophysica acta},
  year={1992},
  volume={1159 2},
  pages={
          113-21
        }
}
The guinea-pig and human Hageman factors differ in their sensitivity to activation by particular bacterial proteinases. To understand this difference, the primary structure and cleavage site on activation of the guinea-pig molecule were determined and compared with the human molecule. By the use of a synthetic oligodeoxyribonucleotide probe which encoded a part of human Hageman factor cDNA, a cDNA clone was isolated from a lambda gt11 cDNA library of guinea-pig liver and sequenced. The cDNA… CONTINUE READING