Primary structure of chicken liver dihydrofolate reductase.

  title={Primary structure of chicken liver dihydrofolate reductase.},
  author={Aaram A. Kumar and D. T. Blankenship and B T Kaufman and J H Freisheim},
  volume={19 4},
The complete covalent structure of dihydrofolate reductase from chicken liver is described. The S-carboxymethylated protein was subjected to cleavage by cyanogen bromide which produced five fragments. Fragment CB2 contained an internal homoserine residue which was not cleaved by cyanogen bromide. Sequences and ordering of the cyanogen bromide fragments were established by means of automated sequencer analyses of the fragments and from smaller peptides generated by proteolysis with trypsin and… CONTINUE READING