Primary structure of a squid acid and base non-specific ribonuclease.

  title={Primary structure of a squid acid and base non-specific ribonuclease.},
  author={Aaron Kusano and M. Iwama and Kazuko Ohgi and Masachika Irie},
  journal={Bioscience, biotechnology, and biochemistry},
  volume={62 1},
Squid (Todarodes pacificus) liver RNase (RNase Tp) was purified. RNase Tp was a base non-specific and acid RNase. Upon hydrolysis of RNA, RNase Tp released four mononucleotides in the order of G > A > U > C. RNase Tp consisted of two peptides with 198 and 23 amino acid residues. The amino acid sequences of these peptides were analyzed. The large peptide had two unique segments containing most of the active site amino acid residues of RNase T2 family enzymes. From the comparison of the sequence… CONTINUE READING

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