Primary structure of Lep d I, the main Lepidoglyphus destructor allergen.

Abstract

The most relevant allergen of the storage mite Lepidoglyphus destructor (Lep d I) has been characterized. Lep d I is a monomer protein of 13273 Da. The primary structure of Lep d I was determined by N-terminal Edman degradation and partially confirmed by cDNA sequencing. Sequence polymorphism was observed at six positions, with non-conservative substitutions in three of them. No potential N-glycosylation site was revealed by peptide sequencing. The 125-residue sequence of Lep d I shows approximately 40% identity (including the six cysteines) with the overlapping regions of group II allergens from the genus Dermatophagoides, which, however, do not share common allergenic epitopes with Lep d I.

Cite this paper

@article{Varela1994PrimarySO, title={Primary structure of Lep d I, the main Lepidoglyphus destructor allergen.}, author={Jos{\'e} Ignacio Varela and P Ventas and Jo227o Carreira and Julio A. Barbas and Guillermo Gim{\'e}nez-Gallego and Florentino Polo}, journal={European journal of biochemistry}, year={1994}, volume={225 1}, pages={93-8} }