Primary structure and mitochondrial import in vitro of the 20.9 kDa subunit of complex I from Neurospora crassa.

@article{Azevedo1992PrimarySA,
  title={Primary structure and mitochondrial import in vitro of the 20.9 kDa subunit of complex I from Neurospora crassa.},
  author={Jo{\~a}o Valle e Azevedo and U. Nehls and Christoph Eckerskorn and Hendrikje Heinrich and Helga Rothe and Hanns Weiss and Sigurd Werner},
  journal={The Biochemical journal},
  year={1992},
  volume={288 ( Pt 1)},
  pages={29-34}
}
The 20.9 kDa subunit of NADH:ubiquinone oxidoreductase (complex I) from Neurospora crassa is a nuclear-coded component of the hydrophobic arm of the enzyme. We have determined the primary structure of this subunit by sequencing a full-length cDNA and a cleavage product of the isolated polypeptide. The deduced protein sequence is 189 amino acid residues long and contains a putative membrane-spanning domain. Striking similarity over a 60 amino-acid-residue domain with the M (matrix) protein of… CONTINUE READING