Primary structure and functional expression of rat and human stem cell factor DNAs

  title={Primary structure and functional expression of rat and human stem cell factor DNAs},
  author={Francis H. Martin and Sidney V. Suggs and Keith E. Langley and Hsieng S. Lu and Jerry Ting and Kenneth H. Okino and Charles F. Morris and Ian K. Mcniece and Frederick W. Jacobsen and Elizabeth A. Mendlaz and Neal C. Birkett and Kent Andrew Smith and Merrie Jo Johnson and Vann P. Parker and Josephine C. Flores and A C Patel and Eric F. Fisher and Holly O. Erjavec and C. J. Baquero Herrera and Jette Wypych and Raj K. Sachdev and Joseph A. Pope and Ian Leslie and Duanzhi Wen and Chi-Hwei Lin and Rod L. Cupples and Krisztina Maria Zsebo},
Biological characteristics of recombinant rat and human stem cell factor
The rate of recovery of bone marrow progenitors in the SCF-treated mice was faster than in the controls, with a small difference in the total WBC between the two groups during the time (10 days post irradiation) when 90% of control mice and 0% of theSCF- treated mice were dead.
Human Stem Cell Factor: Biological Effects and Receptor Expression on Myeloid Leukemia Cells
The aim of this study was to investigate the mitogenic potential of SCF alone or in synergy with other factors and the pattern of its receptor expression on myeloid leukemia cells.
Porcine stem cell factor/c-kit ligand: its molecular cloning and localization within the uterus.
Pregnancy does not appear to be a prerequisite for pSCF mRNA expression in endometrial tissue since it was detectable in tissue and tcRNA obtained from pregnant and nonpregnant gilts, but it probably participates in intracellular communication within the uterus.
Enhancement of murine blast cell colony formation in culture by recombinant rat stem cell factor, ligand for c-kit.
The effects of recombinant rat SCF (rrSCF) on colony formation from primitive hematopoietic progenitors in culture and combinations of factors indicated that rrSCF, like IL-6, G-CSF, and IL-11, shortens the dormant period in which the stem cells reside.
Structure-function relationships of stem cell factor: an analysis based on a series of human-murine stem cell factor chimera and the mapping of a neutralizing monoclonal antibody.
Findings remarkably similar to previous studies of cytokines as diverse as growth hormone, GM-CSF, and interleukin (IL)-4 suggest that cytokines of multiple classes share a common functional organization.


Molecular cloning of a complementary DNA encoding human macrophage-specific colony-stimulating factor (CSF-1).
The CSF-1 appears to be encoded by a single-copy gene, but its expression results in the synthesis of several messenger RNA species, ranging in size from about 1.5 to 4.5 kilobases.
Cloning and expression of the human erythropoietin gene.
  • F. Lin, S. Suggs, Z. Stabinsky
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1985
The human erythropoietin gene has been isolated from a genomic phage library by using mixed 20-mer and 17-mer oligonucleotide probes and encodes a 27-amino acid signal peptide and a 166-AMino acid mature protein with a calculated Mr of 18,399.
High-efficiency cloning of full-length cDNA.
The cloning procedure described here mitigates this shortcoming by attributing the high efficiency of cloning full- or nearly full-length cDNA to the fact that the plasmid DNA vector itself serves as the primer for first- and second-strand cDNA synthesis.
Colony-forming cells with high proliferative potential (HPP-CFC).
These properties suggest that the HPP-CFC represent an important cell type in hematopoiesis and provide a model system, particularly in the human, for studying the properties of primitive progenitor cells in vitro.
Effects of hematopoietin-1 and interleukin 1 activities on early hematopoietic cells of the bone marrow.
The results indicate that the IL 1 alpha target cell population is different than the target cell populations of IL 3, granulocyte-macrophage colony-stimulating factor, and that IL 1Alpha has at least a survival-enhancing and possibly proliferation-inducing effect on primitive hematopoietic stem cells.
SR alpha promoter: an efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter and the R-U5 segment of human T-cell leukemia virus type 1 long terminal repeat.
The SR alpha expression system was 1 or 2 orders of magnitude more active than the SV40 early promoter in a wide variety of cell types, including fibroblasts and lymphoid cells, and was capable of promoting a high level of expression of various lymphokine cDNAs.