Primary structure and functional expression of a mammalian skeletal muscle sodium channel

@article{Trimmer1989PrimarySA,
  title={Primary structure and functional expression of a mammalian skeletal muscle sodium channel},
  author={James S. Trimmer and Sharon S. Cooperman and Sally A. Tomiko and Jiuying Zhou and Shelia M. Crean and Mary B. Boyle and Roland G. Kalen and Zuhang Sheng and Robert L. Barchi and Fred J. Sigworth and Richard H. Goodman and William S. Agnew and Gail Mandel},
  journal={Neuron},
  year={1989},
  volume={3},
  pages={33-49}
}
We describe the isolation and characterization of a cDNA encoding the alpha subunit of a new voltage-sensitive sodium channel, microI, from rat skeletal muscle. The 1840 amino acid microI peptide is homologous to alpha subunits from rat brain, but, like the protein from eel electroplax, lacks an extended (approximately 200) amino acid segment between homologous domains I and II. Northern blot analysis indicates that the 8.5 kb microI transcript is preferentially expressed in skeletal muscle… Expand
Expression of the sodium channel β 1 subunit in rat skeletal muscle is selectively associated with the tetrodotoxin-sensitive α subunit isoform
Abstract Transcripts homologous to the rat brain sodium channel β subunit ( β 1 ) are prominently expressed in both innervated and denervated adult skeletal muscle and in heart, but not in neonatalExpand
Primary structure and functional expression of a developmentally regulated skeletal muscle chloride channel
TLDR
expression from cRNA in Xenopus oocytes leads to 9-anthracene-carboxylic acid-sensitive currents with time and voltage dependence typical for macroscopic muscle Cl− conductance, and the functional destruction of this channel in mouse myotonia suggests that the major skeletal muscle chloride channel is cloned. Expand
Functional expression and properties of the human skeletal muscle sodium channel
TLDR
The Na+ currents of hSkM1 had abnormally slow inactivation kinetics in oocytes, but relatively normal kinetics when expressed in the mammalian cell line, and the potency of TTX and μCTX is 22-fold lower in h SkM1, primarily due to a higher rate of toxin dissociation. Expand
Cloning of a sodium channel alpha subunit from rabbit Schwann cells.
TLDR
The results of reverse transcriptase-polymerase chain reaction experiments suggest that expression of Nas transcripts is restricted to cells in the peripheral and central nervous systems. Expand
Modification of the Na+ current conducted by the rat skeletal muscle α subunit by coexpression with a human brain β subunit
TLDR
It is shown that a β subunit derived from human brain directs the same functional modification of INa expressed by a rat skeletal muscle α subunit, which implies that functional domains for the interaction of α and β subunits are highly conserved across both tissues and species. Expand
Sequence and genomic structure of the human adult skeletal muscle sodium channel alpha subunit gene on 17q.
TLDR
The amino acid sequence of the sodium channel alpha subunit from adult human skeletal muscle has been deduced by cross-species PCR-mediated cloning and sequencing of the cDNA, providing valuable information for the study of periodic paralysis and paramyotonia congenita, two inherited neurological disorders which are caused by point mutations within this gene. Expand
Gating mechanism of a cloned potassium channel expressed in frog oocytes and mammalian cells
TLDR
A simple model is presented that incorporates the findings of cloned cDNA coding for a delayed rectifier K+ channel from rat brain and rat muscle and is consistent with the presumed structural symmetry of a functional K+ channels. Expand
Primary structure and expression of a sodium channel characteristic of denervated and immature rat skeletal muscle
TLDR
It is suggested that SkM2 is a TTX-insensitive sodium channel expressed in both skeletal and cardiac muscle, similar to other recently cloned sodium channels from rat skeletal muscle. Expand
Functional Co-expression of the 1 and Type IIA Subunits of Sodium Channels in a Mammalian Cell Line (*)
TLDR
It is shown that the Chinese hamster cell lines CHO and 1610 do not express endogenous β1 subunits as determined by Northern blotting, immunoblotting, and assay for α1 subunit function by expression of cellular mRNA in Xenopus oocytes, and that α subunits expressed alone in stable lines of these cells activate and inactivate rapidly. Expand
Primary structure of the adult human skeletal muscle voltage‐dependent sodium channel
TLDR
The cloning and nucleotide sequence determination of the normal product of the SCN4A gene product is reported, which is the human homologue of rSkM1, the tetrodotoxin‐sensitive sodium channel characteristic of adult rat skeletal muscle. Expand
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TLDR
It is reported that the messenger RNAs generated by transcription of the cloned cDNAs encoding the rat brain sodium channel large polypeptides, when injected into Xenopus oocytes, can direct the formation of functional sodium channels. Expand
A rat brain na+ channel α subunit with novel gating properties
TLDR
A full-length rat brain Na + channel α subunit cDNA is constructed that differs from the previously reported a subunit of Noda et al. at 6 amino acid positions indicating the presence of a component, either a structural sub unit of the channel complex or a modifying enzyme, necessary for normal gating of the channels. Expand
Existence of distinct sodium channel messenger RNAs in rat brain
The sodium channel is a voltage-gated ionic channel essential for the generation of action potentials1–3. It has been reported that the sodium channels purified from the electric organ ofExpand
Messenger RNA coding for only the alpha subunit of the rat brain Na channel is sufficient for expression of functional channels in Xenopus oocytes.
  • A. L. Goldin, T. Snutch, +7 authors R. Dunn
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1986
TLDR
Results demonstrate that mRNA encoding the alpha subunit of the rat brain Na channel, in the absence of any beta-subunit mRNA, is sufficient for translation to give functional channels in oocytes. Expand
Modulation of sodium-channel mRNA levels in rat skeletal muscle.
TLDR
It is found that Na-channel mRNA can be detected as early as embryonic day 17 and that mRNA levels increase 2- to 3-fold between birth and postnatal day 35, suggesting that expression of Na- channel genes in fast-twitch muscle may be regulated by the state of innervation. Expand
The voltage-sensitive sodium channel from rabbit skeletal muscle. Chemical characterization of subunits.
TLDR
Using the isolated subunits, the protein-labeling system was calibrated on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and the subunit stoichiometry for the rabbit skeletal muscle channel was determined; in the native preparation, the molar ratio of alpha:beta is 1 : 1. Expand
Structure of the voltage‐dependent potassium channel is highly conserved from Drosophila to vertebrate central nervous systems.
TLDR
Structural conservation of domains of the deduced protein indicate that the rat brain cDNA encodes a voltage‐sensitive potassium channel, and 82% is homologous to the Drosophila Shaker protein indicating that voltage‐ sensitive potassium channels have been highly conserved during evolution. Expand
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TLDR
The isolation of complementary DNA clones from the mouse brain are reported, the nucleotide sequences of which predict a protein remarkably similar to the Shaker protein, suggesting that these mouse clones encode a potassium channel component and that the conserved amino acids may be essential to some aspect of potassium channel function. Expand
Primary structure of the receptor for calcium channel blockers from skeletal muscle
TLDR
Structural and sequence similarities to the voltage-dependent sodium channel suggest that in the transverse tubule membrane of skeletal muscle the dihydropyridine receptor may act both as voltage sensor in excitation-contraction coupling and as a calcium channel. Expand
Purification and functional reconstitution of the voltage-sensitive sodium channel from rabbit T-tubular membranes.
TLDR
The voltage-sensitive sodium channel has been purified from rabbit T-tubular membranes and reconstituted into defined phospholipid vesicles and retained the ability to gate specific 22Na+ influx in response to activation by batrachotoxin or veratridine. Expand
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