Primary structure analysis and functional expression of erythrose reductases from erythritol-producing fungi (Trichosporonoides megachiliensis SNG-42).

@article{Ookura2005PrimarySA,
  title={Primary structure analysis and functional expression of erythrose reductases from erythritol-producing fungi (Trichosporonoides megachiliensis SNG-42).},
  author={Tetsuya Ookura and Keiko Azuma and Kenji Isshiki and Hajime Taniguchi and Takafumi Kasumi and Yukio Kawamura},
  journal={Bioscience, biotechnology, and biochemistry},
  year={2005},
  volume={69 5},
  pages={944-51}
}
NADPH-dependent erythrose reductases (ERs) in erythritol-producing fungi, Trichosporonoides megachiliensis SNG-42, catalyze the reduction of D-erythrose. We previously characterized the biochemical properties of three isozymes of ERs (ER-I, ER-II, and ER-III). Using internal amino acid sequences of ER-III and ER-I with peptide mapping, we cloned three cDNAs (er1, 1121-bp (AB191474); er2, 1077-bp (AB191475); er3, 1119-bp (AB191476)). The er3 cDNA encoded a polypeptide 36,044 Da, and its deduced… CONTINUE READING