Primary human CD34+ hematopoietic stem and progenitor cells express functionally active receptors of neuromediators.

@article{Steidl2004PrimaryHC,
  title={Primary human CD34+ hematopoietic stem and progenitor cells express functionally active receptors of neuromediators.},
  author={Ulrich G. Steidl and Simone Bork and Sebastian Schaub and Oliver Selbach and Janette {\vS}ere{\vs} and Manuel A Aivado and Thomas Schroeder and Ulrich Peter Rohr and Roland Fenk and Slawomir Kliszewski and Christian Maercker and Peter Neubert and Stefan R. Bornstein and Helmut L. Haas and Guido Kobbe and Daniel Geoffrey Tenen and Rainer Haas and Ralf Kronenwett},
  journal={Blood},
  year={2004},
  volume={104 1},
  pages={
          81-8
        }
}
Recently, overlapping molecular phenotypes of hematopoietic and neuropoietic cells were described in mice. Here, we examined primary human CD34(+) hematopoietic stem and progenitor cells applying specialized cDNA arrays, real-time reverse-transcriptase-polymerase chain reaction (RT-PCR), and fluorescent-activated cell sorter (FACS) analysis focusing on genes involved in neurobiologic functions. We found expression of vesicle fusion and motility genes, ligand- and voltage-gated ion channels… 

Figures and Tables from this paper

Blood-forming stem cells are nervous: Direct and indirect regulation of immature human CD34+ cells by the nervous system

The neurotransmitter GABA is a potent inhibitor of the stromal cell-derived factor-1alpha induced migration of adult CD133+ hematopoietic stem and progenitor cells.

The neurotransmitter GABA is a potent blocker of the SDF-1alpha-induced migration of CD133(+) HSPCs from mobilized peripheral blood, and results suggest that GABA(B)-receptor signaling modulates the activity of CRAC channels, whereby the mechanism in detail remains unclear.

Neurexophilin 1 suppresses the proliferation of hematopoietic progenitor cells.

The results demonstrate the presence and function of a regulated signaling axis in hematopoiesis centered on NRXN1α and its modulation by DAG1 and NXPH1.

The neurotransmitter receptor Gabbr1 regulates proliferation and function of hematopoietic stem and progenitor cells.

A direct role for GABBR1 in HSPC proliferation is indicated, a potential target to improve HSPCs engraftment in clinical transplantation is identified, and an ex vivo treatment prior to transplant into immunodeficient mice is performed.

Human cord blood-derived hematopoietic and neural-like stem/progenitor cells are attracted by the neurotransmitter GABA.

GA was found to be a potent chemoattractant of HUCB stem/progenitor cells specifically through GABA(B)R activation and was highly enriched by both hematopoietic progenitors and cells able to generate neuron- like cells in culture.

Distinct molecular phenotype of malignant CD34+ hematopoietic stem and progenitor cells in chronic myelogenous leukemia

Gene expression patterns reflected BCR–ABL-induced functional alterations such as increased cell-cycle and proteasome activity and upregulation of the proto-oncogene SKI and of receptors for neuromediators, suggesting that the adenosine A1 receptor is of functional relevance in CML hematopoietic progenitor cells.

The Laminin Receptors Basal Cell Adhesion Molecule/Lutheran and Integrin α7β1 on Human Hematopoietic Stem Cells

Two further specific laminin receptors, namely integrin α7β1 and basal cell adhesion molecule/Lutheran (BCAM/Lu) are shown to be strongly expressed by human lineage-negative CD34 + HSPC and should be taken into consideration in future studies.

Neurological Regulation of the Bone Marrow Niche.

A comprehensive overview is given of their role and interactions with important cells in the hematopoietic niche, including HSPCs and MSCs, and their effect on HSPC maintenance, regulation and mobilization.

Hepatic and pancreatic stellate cells in focus

Findings support the concept that stellate cells are undifferentiated cells, which might play an important role in liver regeneration.
...

References

SHOWING 1-10 OF 67 REFERENCES

Hematopoietic progenitors express neural genes

It is demonstrated here that a subset of ex vivo bone marrow cells expresses the neurogenic transcription factor Pax-6 as well as neuronal genes encoding neurofilament H, NeuN (neuronal nuclear protein), HuC/HuD (Hu-antigen C/Hu-Antigen D), and GAD65 (glutamic acid decarboxylase 65) and the oligodendroglial gene encoding CNPase can be regulated in brain.

Gene expression profiling identifies significant differences between the molecular phenotypes of bone marrow-derived and circulating human CD34+ hematopoietic stem cells.

The data molecularly confirm and explain the finding that CD34+ cells residing in the bone marrow cycle more rapidly, whereas circulating CD34+, consisting of a higher number of quiescent stem and progenitor cells, provides novel molecular insight into stem cell physiology.

From hematopoiesis to neuropoiesis: Evidence of overlapping genetic programs

It is proposed that at least some of the transcripts that are selectively and commonly expressed in two or more types of stem cells define a functionally conserved group of genes evolved to participate in basic stem cell functions, including stem cell self-renewal.

Neurohormones and Catecholamines as Functional Components of the Bone Marrow Microenvironment

  • G. Maestroni
  • Biology
    Annals of the New York Academy of Sciences
  • 2000
It is demonstrated that pre‐B cells express α1B‐adrenoceptors and that their activation by catecholamines results in suppressed myelopoiesis in vitro or protection in vivo against supralethal doses of carboplatin.

Expression of two inward rectifier potassium channels is essential for differentiation of primitive human hematopoietic progenitor cells

Inhibition of the expression of each of the channels suppressed progenitor cell generation by IL‐3 and SCF‐stimulated CD34+38− cells in 7‐day suspension cultures, suggesting a potentially important role in potassium homeostasis in these cells.

The Role of Cytokines and Adhesion Molecules for Mobilization of Peripheral Blood Stem Cells

The role of hematopoietic growth factors, chemokines, and adhesion molecules for mobilization and homing of CD34+ cells is summarized and an overview of new classes of mobilizing drugs such as monoclonal antibodies, specific peptides, or antisense oligonucleotides targetingAdhesion molecules are described.

Bone Marrow as a Source of Endothelial Cells and NeuN-Expressing Cells After Stroke

Bone marrow–derived cells are a source of endothelial cells and NeuN-expressing cells after cerebral infarction and this plasticity may be exploited in the future to enhance recovery after stroke.

Altered Hematopoiesis, Behavior, and Sexual Function in μ Opioid Receptor–deficient Mice

A novel role of the μ opioid receptor is suggested in hematopoiesis and reproductive physiology, in addition to its known involvement in pain relief.
...