• DOI:10.1182/BLOOD-2004-01-0373
• Corpus ID: 25679667

Primary human CD34+ hematopoietic stem and progenitor cells express functionally active receptors of neuromediators.

@article{Steidl2004PrimaryHC,
  title={Primary human CD34+ hematopoietic stem and progenitor cells express functionally active receptors of neuromediators.},
  author={Ulrich G. Steidl and Simone Bork and Sebastian Schaub and Oliver Selbach and Janette {\vS}ere{\vs} and Manuel A Aivado and Thomas Schroeder and Ulrich Peter Rohr and Roland Fenk and Slawomir Kliszewski and Christian Maercker and Peter Neubert and Stefan R. Bornstein and Helmut L. Haas and Guido Kobbe and Daniel Geoffrey Tenen and Rainer Haas and Ralf Kronenwett},
  journal={Blood},
  year={2004},
  volume={104 1},
  pages={
          81-8
        }
}

• U. Steidl, S. Bork, +15 authors R. Kronenwett
• Published 1 July 2004
• Biology
• Blood

Recently, overlapping molecular phenotypes of hematopoietic and neuropoietic cells were described in mice. Here, we examined primary human CD34(+) hematopoietic stem and progenitor cells applying specialized cDNA arrays, real-time reverse-transcriptase-polymerase chain reaction (RT-PCR), and fluorescent-activated cell sorter (FACS) analysis focusing on genes involved in neurobiologic functions. We found expression of vesicle fusion and motility genes, ligand- and voltage-gated ion channels, receptor kinases and phosphatases, and, most interestingly, mRNA as well as protein expression of G protein-coupled receptors of neuromediators (corticotropin-releasing hormone 1 [CRH 1] and CRH 2 receptors, orexin/hypocretin 1 and 2 receptors, GABAB receptor, adenosine A(2)B receptor, opioid kappa 1 and mu 1 receptors, and 5-HT 1F receptor). As shown by 2-color immunofluorescence, the protein expression of these receptors was higher in the more immature CD38(dim) than in the CD38(bright) subset within the CD34(+) population, and completely absent in fully differentiated blood cells, suggesting that those receptors play a role in developmentally early CD34(+) stem and progenitor cells. The intracellular concentration of cyclic adenosine monophosphate (cAMP) in CD34(+) cells was diminished significantly upon stimulation of either CRH or orexin receptors, indicating that those are functionally active and coupled to inhibitory G proteins in human hematopoietic cells. In conclusion, these findings suggest a molecular interrelation of neuronal and hematopoietic signaling mechanisms in humans.