Primary epithelial cell models for cystic fibrosis research.

@article{Randell2011PrimaryEC,
  title={Primary epithelial cell models for cystic fibrosis research.},
  author={Scott H. Randell and M. Leslie Fulcher and Wanda O'neal and John Cumming Olsen},
  journal={Methods in molecular biology},
  year={2011},
  volume={742},
  pages={
          285-310
        }
}
When primary human airway epithelial (hAE) cells are grown in vitro on porous supports at an air-liquid interface (ALI), they recapitulate in vivo morphology and key physiologic processes. These cultures are useful for studying respiratory tract biology and diseases and for testing new cystic fibrosis (CF) therapies. This chapter gives protocols enabling creation of well-differentiated primary CF and non-CF airway epithelial cell cultures with non-proprietary reagents. We also discuss the… CONTINUE READING
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