Primary cultures of normal and tumoral human ovarian epithelium: a powerful tool for basic molecular studies.

@article{Lounis1994PrimaryCO,
  title={Primary cultures of normal and tumoral human ovarian epithelium: a powerful tool for basic molecular studies.},
  author={Hafida Lounis and Diane Mich{\`e}le Provencher and Charles Godbout and Daniel Fink and Martin Milot and A. M. Mes-Masson},
  journal={Experimental cell research},
  year={1994},
  volume={215 2},
  pages={303-9}
}
To begin delineating the cellular and molecular events that are important in ovarian carcinogenesis, we have developed a simple and rapid method for the establishment of primary cultures derived from benign tumors, malignant tumors, and ascites of the ovary that are representative of the original clinical material from which they are derived. From 23 ovarian epithelial ascites collected, 13 were successfully established in culture and cells survived an average of 7 to 8 passages. From 65 solid… CONTINUE READING

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