Primary cultures of human hepatocytes but not HepG2 hepatoblastoma cells are suitable for the study of glycosidic conjugation of bile acids.

@article{Ellis2001PrimaryCO,
  title={Primary cultures of human hepatocytes but not HepG2 hepatoblastoma cells are suitable for the study of glycosidic conjugation of bile acids.},
  author={Ewa Ellis and Elke Roeb and Hanns-Ulrich Marschall},
  journal={Biochimica et biophysica acta},
  year={2001},
  volume={1530 2-3},
  pages={
          155-61
        }
}
Mice lacking Mrp3 (Abcc3) have normal bile salt transport, but altered hepatic transport of endogenous glucuronides.
Profiling of urinary bile acids in piglets by a combination of enzymatic deconjugation and targeted LC-MRM-MS[S]
TLDR
Results support that CDCA is a precursor of γ-muricholic acid in BA biosynthesis in piglets, and a method using a combination of enzymatic deconjugation and targeted LC-multiple reaction monitoring (MRM)-MS analysis for analyzing all common bile acids in piglet urine.
From the Department of Molecular Medicine and Surgery Karolinska Institutet, Stockholm, Sweden INVESTIGATION OF HEPATOCYTE SIGNALING PATHWAYS IN CHRONIC KIDNEY DISEASE. CLINICAL AND EXPERIMENTAL STUDIES
TLDR
It is concluded that systemic blood concentrations of FGF-19 can be considered to mirror portal concentrations at least in non-CKD populations, and the role of hepatocytes exposed to uremic sera rapidly develop an unhealthy metabolism characterized by increased gluconeogenesis and lipogenesis accompanied by perturbations of several key cellular signaling networks.

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Human hepatoblastoma cells (HepG2) and rat hepatoma cells are defective in important enzyme activities in the oxidation of the C27 steroid side chain in bile acid formation.
TLDR
It is concluded that HepG2 human hepatoblastoma cells and 7800 C1 Morris rat hepatoma cells have defects in two enzyme activities involved in the peroxisomal oxidation in bile acid formation, the microsomal THCA-CoA ligase and the per oxisomal ThCA- coA oxidase.
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C cultured HepG2 cells synthesize bile acid, but in a pattern distinct from that of adult human liver, which suggests that this cell line may be a model for studying pathways of human bile acids synthesis, conjugation, and sulfation.
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    Proceedings of the National Academy of Sciences of the United States of America
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TLDR
The results suggest that a glucoside-conjugation pathway of bile acids exists in humans and is catalyzed by a sugar nucleotide-independent glucosyltransferase and is therefore distinct from the known mechanisms of glycoside conjugation.
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TLDR
It is evident that while hormonal factors appear to regulate bile acid synthesis in the rat, no evidence for this was found in human hepatocytes, and this represents a useful model for further studies of the synthesis and regulation of bile acids.
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TLDR
The results suggest that the observed intermediates reflect an alternative biosynthetic pathway to CA, which may be quantitatively significant in the cells.
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TLDR
Therapeutic strategies employing bile acid‐derived cytostatic agents for the treatment of hepatocellular carcinomas may depend upon the expression of the Na+‐independent bile Acid transporter OATP in hepatic malignancies.
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TLDR
Results suggest that the particular subclone of HepG2 cells will be useful for studies of the regulation of bile salt synthesis, but not of transport, by human liver—derived tissue.
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