Primary cultures of human blood-born macrophages grown on hydrophobic teflon membranes.

@article{Andreesen1983PrimaryCO,
  title={Primary cultures of human blood-born macrophages grown on hydrophobic teflon membranes.},
  author={Reinhard Andreesen and J Picht and Georg W. L{\"o}hr},
  journal={Journal of immunological methods},
  year={1983},
  volume={56 3},
  pages={295-304}
}
Human blood-born monocytes have been cultivated on the hydrophobic side of Teflon foils (fluorinated ethylene propylene copolymer) using human pooled AB-serum as essential growth factor. At any stage of culture these in vitro maturing macrophages can easily be detached from the Teflon membrane and subjected to further experimentation. Once established, primary macrophage cultures can be maintained in medium containing 10% FCS for up to 3 months. Lysozyme secretion increased more than 10-fold… CONTINUE READING

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