Primary cultures of dissociated sympathetic neurons. I. Establishment of long-term growth in culture and studies of differentiated properties.

@article{Mains1973PrimaryCO,
  title={Primary cultures of dissociated sympathetic neurons. I. Establishment of long-term growth in culture and studies of differentiated properties.},
  author={Richard E Mains and Paul H. Patterson},
  journal={The Journal of cell biology},
  year={1973},
  volume={59 2 Pt 1},
  pages={329-45}
}
Rat sympathetic ganglia were disrupted by mechanical agitation to yield dissociated primary neurons, and the conditions for long-term growth in culture of the isolated neurons were examined. The neurons were grown with or without non-neural cells, simply by the addition or deletion of bicarbonate during growth in culture. Fluorescence histochemistry indicated that the isolated neurons contained catecholamines; incubations with radioactive precursors were used to verify the synthesis and… CONTINUE READING
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The biosynthesis of octopamine

  • K. BRANDAU, . J.AXELROD
  • Naunyn-Schmiedebergs Arch. Pharmakol. 273:123.
  • 1972

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