Primary culture of ram epididymal epithelial cells

  title={Primary culture of ram epididymal epithelial cells},
  author={Hank H. Hwang and Mark C. Healey and Yoko Y. Elsner and Alice V. Johnston},
A procedure for the primary culture of epithelial cells harvested from the epididymides of young virgin ram lambs is described. A confluent monolayer of epitheial cells can be produced from cell aggregates isolated from the caput and cauda segments of ram lamb epididymides after trypsin and collagenase digestions. A culture of enriched epididymal epithelial cells is obtained using a selective attachment procedure. Characteristic epithelial cell morphology can be confirmed by phase contrast and… CONTINUE READING

Similar Papers

Loading similar papers…