Primary culture of microvascular endothelial cells from bovine retina

@article{Bowman1982PrimaryCO,
  title={Primary culture of microvascular endothelial cells from bovine retina},
  author={Phillip D. Bowman and Anthony Betz and Gary W. Goldstein},
  journal={In Vitro},
  year={1982},
  volume={18},
  pages={626-632}
}
To provide an in vitro system for studying retinal capillary function we have developed methods for isolation and culture of microvascular endothelial cells from retina. Retinal microvessels were isolated by homogenization of the retina and collection of the microvessels onto nylon mesh. Treatment of the isolated microvessels with collagenase and dispase followed by Percoll gradient centrifugation yielded endothelial cells that were largely free of pericytes. A homogeneous population of… CONTINUE READING

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Characteristics of cultured brain capillaries

  • P. D. Bowman, A. L. Betz, G. W. Goldstein
  • J. Cell. Biol
  • 1979

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