Primary culture of lymphoid organ cells and haemocytes of kuruma shrimp, Penaeus japonicus.

@article{Itami1999PrimaryCO,
  title={Primary culture of lymphoid organ cells and haemocytes of kuruma shrimp, Penaeus japonicus.},
  author={Toshiaki Itami and Mizuki Maeda and Mitsuko Kondo and Yoshihiko Takahashi},
  journal={Methods in cell science : an official journal of the Society for In Vitro Biology},
  year={1999},
  volume={21 4},
  pages={237-44}
}
A primary cell culture system was developed for the cells of lymphoid organ tissue of kuruma shrimp, Penaeus japonicus. Minced tissues of lymphoid organs were seeded and incubated at 30 degrees C in medium 199 supplemented with 20% foetal bovine serum, a salt mixture and a lactalbumin hydrolysate (0.1 g/l). Fibroblast-like cells and epithelioid-like cells survived for 54 days. Cells did not survive after trypsin, collagenase or hyaluronidase treatment used for cell dissociation. Mitogens (Con A… CONTINUE READING