Primary culture of hemocytes from Eriocheir sinensis and their immune effects to the novel crustacean pathogen Spiroplasma eriocheiris

@article{Liang2012PrimaryCO,
  title={Primary culture of hemocytes from Eriocheir sinensis and their immune effects to the novel crustacean pathogen Spiroplasma eriocheiris},
  author={Tingming Liang and Hong Tai Ji and Jie Du and Jiangtao Ou and Wen Jie Li and Ting Wu and Qing-guo Meng and Wei Gu and Wen Wang},
  journal={Molecular Biology Reports},
  year={2012},
  volume={39},
  pages={9747-9754}
}
To investigate the interaction between Chinese mitten crab Eriocheir sinensis hemocytes and the pathogen Spiroplasma eriocheiris, a system for in vitro culture of E. sinensis hemocytes with high viability was developed. Following optimization of conditions, hemocytes survived for >35 days. After challenge with the novel crustacean pathogen S. eriocheiris, E. sinensis hemocytes began to develop vacuoles, and then they began to die (within 60 h). Real-time RT-PCR analysis of S. eriocheiris… CONTINUE READING

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