Primary culture of chondrocytes embedded in collagen gels.

  title={Primary culture of chondrocytes embedded in collagen gels.},
  author={Natsuo Yasui and Susumu Osawa and Takahiro Ochi and Hideki Nakashima and Koichiro Ono},
  journal={Experimental cell biology},
  volume={50 2},
Chondrocytes isolated from sternal cartilage of 13-day embryonic chicks were dispersively embedded inside the collagen gels in Ham's F-12 medium containing fetal calf serum. Viability, three-dimensional growth and deposition of extracellular matrix in the living cultures were evidenced by increased cell number, morphology and autoradiographic observations, respectively. The cells increased 2- to 3-fold in 1 week and occupied ' chondrocytic lacunae' surrounded by 'territorial matrix'. A more… CONTINUE READING