Primary culture of bovine chromaffin cells

@article{OConnor2007PrimaryCO,
  title={Primary culture of bovine chromaffin cells},
  author={Daniel T. O'Connor and Sushil K Mahata and Manjula Mahata and Qijiao Jiang and Vivian Hook and Laurent Taupenot},
  journal={Nature Protocols},
  year={2007},
  volume={2},
  pages={1248-1253}
}
This protocol describes the primary culture of individual chromaffin cells derived by enzymatic digestion from the adrenal medulla of the bovine adrenal gland. Since the late 1970s, such cells have provided a useful model system to study neurotransmitter biosynthesis, storage and release in the catecholaminergic system. The protocol can be divided into three stages: isolation of cells (4–6 h), determination of viable cell numbers (approximately 30 min) and growth in culture (3–7 d). An… CONTINUE READING
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