Primary chondrocytes enhance cartilage tissue formation upon co-culture with a range of cell types

  title={Primary chondrocytes enhance cartilage tissue formation upon co-culture with a range of cell types},
  author={Jeanine Anna Alphonse Hendriks and Razvan L. Miclea and Roka Schotel and Ewart de Bruijn and Lorenzo Moroni and Marcel Karperien and Jens Uwe Riesle and Clemens A. van Blitterswijk},
  journal={Soft Matter},
Co-culture models have been increasingly used in tissue engineering applications to understand cell–cell interactions and consequently improve regenerative medicine strategies. Aiming at further elucidating cartilage tissue formation, we co-cultured bovine primary chondrocytes (BPCs) with human expanded chondrocytes (HECs), human dermal fibroblasts (HDFs), mouse embryonic stem cells (MESCs), or mouse-3T3 feeder cells (M3T3s) in micromasses. BPCs were either co-cultured (1:5 ratio) with all cell… 

Progress of co-culture systems in cartilage regeneration

Co-culture system is proven to address many issues encountered by monocultures in cartilage tissue engineering, including reducing the number of chondrocytes needed and alleviating the dedifferentiation of chonds, in order to solve the difficult problem regarding the regeneration of functional cartilage.

Trophic effects of mesenchymal stem cells increase chondrocyte proliferation and matrix formation.

It is clearly demonstrated that in pellet cocultures of MSCs and PCs, the former cells disappear over time, mainly due to a trophic role of the M SCs in stimulating chondrocyte proliferation and matrix deposition by chONDrocytes rather than MSCS actively undergoing chondrogenic differentiation.

Direct Cell-Cell Contact with Chondrocytes Is a Key Mechanism in Multipotent Mesenchymal Stromal Cell-Mediated Chondrogenesis.

Results suggest that MSCs stimulate cartilage formation when placed in close proximity to chondrocytes and that direct cell-cell contact and communication through gap junctions are essential in this chondROinductive interplay.

Trophic effects of mesenchymal stem cells in chondrocyte co-cultures are independent of culture conditions and cell sources.

Results clearly demonstrate that in co-culture pellets, the MSCs stimulate cartilage formation due to a trophic effect on the chondrocytes rather than differentiating into chondROcytes, irrespective of culture condition or origin.

Trophic effects of adipose-tissue-derived and bone-marrow-derived mesenchymal stem cells enhance cartilage generation by chondrocytes in co-culture

It is demonstrated that replacing 80% of bACs by either hAMSCs or hBMSCs does not influence cartilage matrix production or stability, and the remaining chondrocytes produce more matrix due to trophic factors produced by hMSCs.

Engineering Cartilage Tissue by Co-culturing of Chondrocytes and Mesenchymal Stromal Cells.

This chapter provides detailed protocols for analyzing MSC-chondrocyte co-cultures with cell tracking, proliferation assays, species-specific polymerase chain reactions (PCR), rheological analysis, compression analysis, RNA-sequencing analysis, short tandem repeats analysis, and biochemical examination.

Advances in tissue engineering through stem cell‐based co‐culture

In this review, critical aspects of stem cell use in co‐culture systems are discussed, and direct and indirect co-culture methodologies used in tissue engineering are described, along with various characteristics of cellular interactions in these systems.

Improving In Vitro Cartilage Generation by Co-Culturing Adipose-Derived Stem Cells and Chondrocytes on an Allograft Adipose Matrix Framework

This novel model of cartilage engineering provides a setting for using the patient’s own chondrocytes and adipose tissue to create a customized ear framework that could be further used for surgical reconstruction.

Fibroblast growth factor-1 is a mesenchymal stromal cell-secreted factor stimulating proliferation of osteoarthritic chondrocytes in co-culture.

It is demonstrated that MSCs increase FGF-1 secretion on co-culture with hPCs, which, in turn, is responsible for increased h PCs proliferation in pellet co-cultures.

Mesenchymal stromal/stem cell-or chondrocyte-seeded microcarriers as building blocks for cartilage tissue engineering.

This is the first article that successfully combines an innovative combination of cell expansion on microcarrier and the direct use of MSC- or hch-cell-laden microcarriers as building blocks in cartilage tissue engineering.

In vitro cartilage tissue formation by Co-culture of primary and passaged chondrocytes.

There was an increase in cartilage tissue formation with the addition of increasing numbers of primary chondrocytes, up to 20%, and this coculture approach could provide a new way to solve the problem of limited cell number for Cartilage tissue engineering.

Chondrogenic Differentiation of Adult Dermal Fibroblasts

RT-PCR for collagen II supports the hypothesis that dermal fibroblasts can be triggered to differentiate by culture on cartilage matrix proteoglycans, and provides an initial step towards a cell source that may prove equally successful for the generation of cartilage in the laboratory.

Co-culture of osteoblasts and chondrocytes modulates cellular differentiation in vitro.

Differentiation of human embryonic stem cells on three-dimensional polymer scaffolds

It is shown that complex structures with features of various committed embryonic tissues can be generated, in vitro, by using early differentiating hES cells and further inducing their differentiation in a supportive 3D environment such as poly(lactic-co-glycolic acid)/poly(l-lactic acid) polymer scaffolds.

Coculture of mesenchymal stem cells and respiratory epithelial cells to engineer a human composite respiratory mucosa.

This in vitro model of respiratory epithelium, which exhibited morphologic, histologic, and functional features of a tracheal mucosa, could help to understand interactions between mesenchymal and epithelial cells and mechanisms involved in mucus production, inflammation, and airway repair.

Modulation of Chondrocytic Properties of Fat-Derived Mesenchymal Cells in Co-Cultures with Nucleus Pulposus

The data suggest that the fat-derived mesenchymal cells responded to soluble mediators from the disk, which suggests that fat tissue may serve as an abundant and easily acquired source of multipotent cells for tissue engineering.

Microfabrication of Hepatocyte/Fibroblast Co‐cultures: Role of Homotypic Cell Interactions

The data suggest that fibroblast number plays a role in modulation of hepatocellular response through homotypic fibro Blast interactions, and this approach will allow further elucidation of the complex interplay between two cell types as they form a functional model tissue in vitro or as they interact in vivo to form afunctional organ.

Dedifferentiation-associated changes in morphology and gene expression in primary human articular chondrocytes in cell culture.

The hypothesis that HACs dedifferentiate when grown in monolayer cultures is supported and the expression patterns show that proliferation and differentiation are exclusive features of human chondrocytes.