Primary Culture of Adult Rat Heart Myocytes

@inproceedings{Xu2009PrimaryCO,
  title={Primary Culture of Adult Rat Heart Myocytes},
  author={Xianghua Xu and Henry M Colecraft},
  booktitle={Journal of visualized experiments : JoVE},
  year={2009}
}
Cultured primary adult rodent heart cells are an important model system for cardiovascular research. Nevertheless, establishment of robust, viable cultured adult myocytes can be a technically challenging, rate-limiting step for many researchers. Here we described a protocol to obtain a high yield of adult rat heart myocytes that remain viable in culture for several days. The heart is isolated and perfused with collagenase and protease under low Ca2+ conditions to recover single myocytes. Ca2… CONTINUE READING

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