Primary Airway Epithelial Cell Gene Editing Using CRISPR-Cas9.


The adaptation of the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR associated endonuclease 9 (CRISPR-Cas9) machinery from prokaryotic organisms has resulted in a gene editing system that is highly versatile, easily constructed, and can be leveraged to generate human cells knocked out (KO) for a specific gene. While standard… (More)
DOI: 10.1007/978-1-4939-7471-9_15


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