Pri sORF peptides induce selective proteasome-mediated protein processing

@article{Zanet2015PriSP,
  title={Pri sORF peptides induce selective proteasome-mediated protein processing},
  author={Jennifer Zanet and E. Benrabah and T Li and Anne P{\'e}lissier-Monier and H{\'e}l{\`e}ne Chanut-Delalande and Brice Ronsin and Hugo J. Bellen and F Payre and Serge Plaza},
  journal={Science},
  year={2015},
  volume={349},
  pages={1356 - 1358}
}
Small peptide regulates protein activity Coding and noncoding RNAs can produce peptides from small open reading frames (smORFs), with a variety of mostly unknown functions. Using a genome-wide screen, Zanet et al. show that Polished rice (Pri) smORF peptides control fruit fly development by binding to an E3 ubiquitin ligase. This changes the ligase's selectivity and triggers proteasome-dependent maturation of the developmental transcription factor Shavenbaby. Other smORF peptides may act by a… 
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References

SHOWING 1-10 OF 41 REFERENCES
Small Peptides Switch the Transcriptional Activity of Shavenbaby During Drosophila Embryogenesis
TLDR
The results demonstrate that during Drosophila embryogenesis, Pri sORF peptides provide a strict temporal control to the transcriptional program of epidermal morphogenesis.
Primary transcripts of microRNAs encode regulatory peptides
TLDR
It is shown that plant pri-miRNAs contain short open reading frame sequences that encode regulatory peptides, suggesting that miPEPs are widespread throughout the plant kingdom and might have agronomical applications.
Small peptide regulators of actin-based cell morphogenesis encoded by a polycistronic mRNA
TLDR
It is demonstrated that pri has essential roles in epithelial morphogenesis by regulating F-actin organization by transcribing into a polycistronic mRNA that contains evolutionarily conserved short ORFs that encode 11 or 32 amino acid-long peptides.
Peptides Encoded by Short ORFs Control Development and Define a New Eukaryotic Gene Family
TLDR
It is shown that tal controls gene expression and tissue folding in Drosophila, thus acting as a link between patterning and morphogenesis, and defines two novel paradigms in eukaryotic coding genes: the existence of short, unprocessed peptides with key biological functions, and their arrangement in polycistronic messengers.
A Human Short Open Reading Frame (sORF)-encoded Polypeptide That Stimulates DNA End Joining*
TLDR
It is shown that a 69-amino acid SEP, MRI-2, physically interacts with the Ku heterodimer to stimulate DNA double-strand break ligation via nonhomologous end joining.
Sequence composition of disordered regions fine-tunes protein half-life
TLDR
It is proposed that the proteasome's sequence preferences provide a second component to the degradation code and may fine-tune protein half-life in cells.
Small open reading frames associated with morphogenesis are hidden in plant genomes
TLDR
The results indicate that many coding sORFs hidden in plant genomes are associated with morphogenesis, and an expression atlas for 16 organs and 17 environmental conditions among 7,901 identified coding s ORFs will contribute to further study of the roles of sORF in plants.
An unstructured initiation site is required for efficient proteasome-mediated degradation
TLDR
The initiation site is identified as a novel component of the targeting signal, which is required to engage the proteasome unfolding machinery efficiently and accelerate proteolysis of tightly folded proteins.
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