Prevention of the cortical reaction in fertilized sea urchin eggs by injection of calcium-chelating ligands.

@article{Zucker1978PreventionOT,
  title={Prevention of the cortical reaction in fertilized sea urchin eggs by injection of calcium-chelating ligands.},
  author={Robert S. Zucker and Richard Anthony Steinhardt},
  journal={Biochimica et biophysica acta},
  year={1978},
  volume={541 4},
  pages={459-66}
}
Eggs from the sea urchin, Lytechinus pictus, were injected with either EGTA or EDTA, and were subsequently fertilized. EGTA prevented cortical vesicle discharge and formation of the fertilization membrane. EDTA had either no effect, or sometimes retarded the elevation of the fertilization membrane, or reduced the percentage of eggs with elevated membranes. Theoretical considerations lead to estimates of the probable effects of EGTA and EDTA on the internally released calcium which triggers the… CONTINUE READING
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