• Corpus ID: 19996844

Prevention of phorbol ester receptor down modulation in human myeloblastic leukemia ML-1 cells by differentiation-stimulating serum components.

@article{Sakagami1984PreventionOP,
  title={Prevention of phorbol ester receptor down modulation in human myeloblastic leukemia ML-1 cells by differentiation-stimulating serum components.},
  author={Hirofumi Sakagami and Rosemary Hromchak and Andreas Block},
  journal={Cancer research},
  year={1984},
  volume={44 8},
  pages={
          3330-5
        }
}
The phorbol esters 12-O-tetradecanoylphorbol-13-acetate, phorbol 12,13-didecanoate, phorbol 12,13-dibutyrate (PDB), and phorbol 12,13-dibenzoate were found to compete with [20-3H]-PDB binding to human myeloblastic leukemia ML-1 cells in approximate proportion to their differentiation-inducing capacity. Fetal bovine serum decreased the down modulation of phorbol ester receptor sites on these cells and increased PDB-induced differentiation. These two activities coeluted upon chromatography of… 

Tables from this paper

A phorbol diester resistant monocytic leukemia cell line is PKC deficient.

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The results indicate that the down regulation of specific [3H]PDB binding may be a crucial early event in the control of phorbol ester-induced terminal differentiation in HL-60 cells and suggest that such down regulation may be involved in other cellular and biochemical effects ofphorbol diester tumor promoters.

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