Prevention of docosahexaenoic acid-induced cytotoxicity by phosphatidic acid in Jurkat leukemic cells: the role of protein phosphatase-1.

@article{Siddiqui2001PreventionOD,
  title={Prevention of docosahexaenoic acid-induced cytotoxicity by phosphatidic acid in Jurkat leukemic cells: the role of protein phosphatase-1.},
  author={Rafat A. Siddiqui and Laura J. Jenski and Jacqueline D Wiesehan and Malcolm Hunter and Richard J. Kovacs and William Stillwell},
  journal={Biochimica et biophysica acta},
  year={2001},
  volume={1541 3},
  pages={188-200}
}
The present investigation explores the role of phosphatidic acid (PA), a specific protein phosphatase-1 (PP1) inhibitor, in cytotoxicity induced by docosahexaenoic acid (DHA). The cytotoxicity of DHA was assayed by quantifying cell survival using the trypan blue exclusion method. A dose-response effect demonstrated that 5 or 10 microM DHA has no effect on Jurkat cell survival; however, 15 microM DHA rapidly decreased cell survival to 40% within 2 h of treatment. Cytotoxicity of 15 microM DHA… CONTINUE READING
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