Methicillin resistant Staphylococcus aureus (MRSA) is a major human pathogen with many clinical aspects. In S. aureus, the accessory gene regulator (agr) globally controls the production of virulence factors. There are four agr groups. Our study was done to define the agr specificity of MRSA circulating at Charles Nicolle hospital and to investigate a possible relationship between agr groups and human disease types. From January 2004 to June 2005, a total of 57 MRSA isolated from individual hospitalized patients were collected, representing 12% of the total S. aureus isolates. The isolates were identified by conventional methods. Methicillin resistance was detected by oxacillin and cefoxitin disks and confirmed by the amplification of mecA gene by PCR. The agr groups were identified by multiplex PCR. All the strains were recovered from different wards: medicine (57.8%) especially from dermatology (56.2%), surgery (28%) and pediatrics (7%). Cutaneous pus (36.84%) and blood culture (35.08%) represented the main specimens. The agr groups were distributed as follow nine (15.7%) belonged to group I, two (3.5%) belonged to group II and 23 (40.3%) belonged to group III. For 23 strains, the agr group was not identified. A relationship between agr group and type of disease was observed: agr group III strains were associated with non invasive infections (P=0.02) and agr group I strains with invasive infections especially bacteremia (P=0.002).