Prevalence of Y chromosome deletions in a Brazilian population of nonobstructive azoospermic and severely oligozoospermic men.

@article{SoPedro2003PrevalenceOY,
  title={Prevalence of Y chromosome deletions in a Brazilian population of nonobstructive azoospermic and severely oligozoospermic men.},
  author={S L S{\~a}oPedro and Renato Fraietta and Deborah Montagnini Spaine and Catarina Segreti Porto and Miguel Srougi and Agnaldo Pereira Cedenho and Maria Christina W Avellar},
  journal={Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas},
  year={2003},
  volume={36 6},
  pages={787-93}
}
We determined the prevalence of Y chromosome deletions in a population of 60 Brazilian nonobstructive azoospermic and severely oligozoospermic men. PCR-based screening of microdeletions was performed on lymphocyte DNA for the presence of 14 sequence-tagged sites (STS) located in the azoospermic factor (AZF) on the Yq chromosome. All STS were amplified efficiently in samples from 12 fertile men tested, but failed to be amplified in samples from fertile women, indicating the specificity of PCR… CONTINUE READING
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