Prevalence of Clinical Isolates of Escherichia coli Producing Inhibitor-Resistant β-Lactamases at a University Hospital in Barcelona, Spain, over a 3-Year Period

  title={Prevalence of Clinical Isolates of Escherichia coli Producing Inhibitor-Resistant $\beta$-Lactamases at a University Hospital in Barcelona, Spain, over a 3-Year Period},
  author={Elisenda Mir{\'o} and Ferr{\'a}n Navarro and Beatriz Mirelis and Montserrat Sabaté and Alba Rivera and Pere Coll and Guillem Prats},
  journal={Antimicrobial Agents and Chemotherapy},
  pages={3991 - 3994}
ABSTRACT About 7% of 7,252 nonduplicated clinical Escherichia coli strains from a Spanish hospital showed reduced susceptibility to amoxicillin-clavulanate. Of these, 0.37% produced the IRTs TEM-30, TEM-31, TEM-33, TEM-34, TEM-37, TEM-40, TEM-51, and TEM-54; 5.3% were probable class C β-lactamase overproducers; 0.8% were probable TEM-1 hyperproducers; 0.18% produced OXA-30; 0.15% overexpressed SHV-1; and 0.03% produced a PSE-1 enzyme. 

Population Analysis and Epidemiological Features of Inhibitor-Resistant-TEM-β-Lactamase-Producing Escherichia coli Isolates from both Community and Hospital Settings in Madrid, Spain

Unlike extended-spectrum β-lactamase producers, the frequency of IRT producers remains low in both community and hospital settings, with most of them causing urinary tract infections.

Spanish Multicenter Study of the Epidemiology and Mechanisms of Amoxicillin-Clavulanate Resistance in Escherichia coli

Although resistant isolates were genetically diverse, clonality was linked to OXA-1-producing isolates of the STs 88 and 131, and the epidemic O25b/B2/ST131 clone carried many different mechanisms of AMC resistance.

Patterns of Resistance to Beta-Lactams and Characterization of Beta-Lactamases in Escherichia coli Isolates from Children in Tunisia

Seventy-six (75.2%) of 101 Escherichia coli isolates, collected over a 2-month period in 2002 at the Children's Hospital of Tunis (Tunisia), were resistant to at least one beta-lactam, which were further characterized by spectrophotometry, isoelectric focusing (IEF) and PCR.

Complexity and diversity of beta-lactamase expression in inhibitor-resistant Escherichia coli from public hospitals in KwaZulu-Natal, South Africa

β-lactamase profles of 38 inhibitor-resistant Escherichia coli isolates obtained from public hospitals in KwaZulu-Natal, selected on the basis of their resistance profles to one/more of amoxicillin/clavulanate, ampicillin/sulbactam and piperacillin/tazobactam detected extensive mobilisation of resistance genes.

High prevalence and diversity of extended-spectrum β-lactamase and emergence of OXA-48 producing Enterobacterales in wildlife in Catalonia

High prevalence of Enterobacterales harbouring a variety of CR genes and OXA-48 mediated-carbapenem resistance, all of them frequently associated to nosocomial human infections, for the first time in wild mammals and wild birds are reported.

Analysis of β-lactamase phenotypes and carriage of selected β-lactamase genes among Escherichia coli strains obtained from Kenyan patients during an 18-year period

The phenotypes and diversity of bla genes in E. coli strains implicated in clinical infections in non-hospitalized and hospitalized patients in Kenya is worryingly high and culture and susceptibility data should guide therapy and surveillance studies for β-lactamase-producers in developing countries.

Molecular Characterization of Resistance to Extended-Spectrum Cephalosporins in Clinical Escherichia coli Isolates from Companion Animals in the United States

This study shows the emergence of conjugative plasmid-borne ESBLs among E. coli strains from companion animals in the United States, which may compromise the effective therapeutic use of ESCs in veterinary medicine.

CTX-M-2 and a New CTX-M-39 Enzyme Are the Major Extended-Spectrum Beta-Lactamases in Multiple Escherichia coli Clones Isolated in Tel Aviv, Israel

The wide dissemination of ESBLs among E. coli isolates in this institution is partly related to clonal spread, but more notably to various plasmid-associated ESBL genes, occurring in multiple clones, wherein the CTX-M gene family appears almost uniformly.



Prevalence of β-Lactamases among 1,072 Clinical Strains of Proteus mirabilis: a 2-Year Survey in a French Hospital

The characterization of the different β-lactamases showed that amoxicillin resistance in P. mirabilis was almost always (97%) associated with TEM or TEM-derived β- lactamase, most of which evolved via T EM-2.

Frequency of inhibitor-resistant TEM beta-lactamases in Escherichia coli isolates from urinary tract infections in France.

DNA-DNA hybridization confirmed that the beta-lactamases involved in this resistance mechanism were TEM-1 derived and contained variations in the altered positions described in IRT enzymes.

Emergence of clinical isolates of Escherichia coli producing TEM-1 derivatives or an OXA-1 beta-lactamase conferring resistance to beta-lactamase inhibitors

Different sets of amino acid substitutions of TEM-1 can be found in clinical isolates and lead to resistance to beta-lactamase inhibitors.

Epidemiological Survey of Amoxicillin-Clavulanate Resistance and Corresponding Molecular Mechanisms in Escherichia coliIsolates in France: New Genetic Features ofblaTEM Genes

Amoxicillin-clavulanate resistance and the corresponding molecular mechanisms were prospectively studied inEscherichia coli over a 3-year period (1996 to 1998) in 14 French hospitals and it was determined that 59 IRT enzymes corresponded to previously described I RT enzymes whereas 8 were new.

Inhibitor-Resistant TEM-33 β-Lactamase in a Shigella sonnei Isolate

The purpose of the present study was to find the molecular basis of the resistance to amoxicillin-clavulanate in the S. sonnei UCK strain and identify the β-lactamase producing this enzyme which is resistant to am toxicillin and ticarcillin.

Molecular characterization of nine different types of mutants among 107 inhibitor-resistant TEM beta-lactamases from clinical isolates of Escherichia coli

Molecular characterization of these mutants showed great diversity among the genes coding for inhibitor-resistant TEM enzymes produced by clinical E. coli isolates, supported by crystallographic data on the TEM-1 enzyme and results of site-directed mutagenesis.

Inhibitor-resistant TEM beta-lactamases: phenotypic, genetic and biochemical characteristics.

The phenotypic, genetic and biochemical characteristics of IRT beta-lactamases are reviewed in an attempt to shed light on the pressures that have contributed to their emergence.

Incidence and mechanisms of resistance to the combination of amoxicillin and clavulanic acid in Escherichia coli

The rarity and diversity of inhibitor-resistant TEM-group enzymes suggest that they are the result of spontaneous mutations that have not yet spread.

Emergence of clinical Escherichia coli isolates with decreased susceptibility to ceftazidime and synergic effect with co-amoxiclav due to SHV-1 hyperproduction.

Enzymatic characterization and the nucleotide sequence confirm the hyperproduction of the SHV-1 enzyme in Escherichia coli according to the disc diffusion test.

Characterization of a new TEM-type beta-lactamase resistant to clavulanate, sulbactam, and tazobactam in a clinical isolate of Escherichia coli

The inhibitory effects of clavulanate, sulbactam, and tazobactam on the TEM-1 enzyme were substantially decreased in comparison with those on IRT-3, as indicated by the 50% inhibitory concentrations.