Prevalence and multiplicity of cutaneous beta papilloma viruses in plucked hairs depend on cellular DNA input.

@article{Weissenborn2009PrevalenceAM,
  title={Prevalence and multiplicity of cutaneous beta papilloma viruses in plucked hairs depend on cellular DNA input.},
  author={Soenke Jan Weissenborn and Rachel Neale and Maurits N. C. de Koning and Tim Waterboer and Damiano C Abeni and J N Bouwes Bavinck and Ulrike Wieland and Herbert J. Pfister},
  journal={Journal of virological methods},
  year={2009},
  volume={161 2},
  pages={280-3}
}
In view of the low loads of beta human papillomaviruses in skin samples, amounts of cellular DNA used in qualitative PCR may become limiting for virus detection and introduce variations in prevalence and multiplicity. This issue was explored within the context of a multicentre study and increasing prevalence and multiplicity was found with increasing input amounts of cellular DNA extracted from hair bulbs. To improve the quality and comparability between different epidemiologic studies ideally… CONTINUE READING