Prevalence and Genetic Diversity of Human Sapoviruses in Shellfish from Commercial Production Areas in Galicia, Spain.

@article{Varela2016PrevalenceAG,
  title={Prevalence and Genetic Diversity of Human Sapoviruses in Shellfish from Commercial Production Areas in Galicia, Spain.},
  author={Miguel F. Varela and David Polo and Jes{\'u}s L Romalde},
  journal={Applied and environmental microbiology},
  year={2016},
  volume={82 4},
  pages={1167-1172}
}
The prevalence of human forms of Sapovirus, an emerging pathogen of human gastroenteritis, was investigated in an 18-month survey from class B mollusc-harvesting areas in two Galician rias (northwest Spain). The detection and quantification of Sapovirus was performed by reverse transcription-real-time PCR, according to the recently developed standard method ISO/TS 15216-1:2013, and genotyping by reverse transcription-nested PCR. The bivalve species studied were wild and cultured mussels… CONTINUE READING