Prevalence and Genetic Diversity Analysis of Human Coronavirus OC43 among Adult Patients with Acute Respiratory Infections in Beijing, 2012

  title={Prevalence and Genetic Diversity Analysis of Human Coronavirus OC43 among Adult Patients with Acute Respiratory Infections in Beijing, 2012},
  author={Q. Hu and R. Lu and Kun-yat Peng and Xijie Duan and Yanqun Wang and Yanjie Zhao and Wen Wang and Y. Lou and W. Tan},
  journal={PLoS ONE},
To determine the prevalence, epidemiology and genetic diversity of human coronavirus OC43 (HCoV-OC43) among adult patients with acute respiratory infections (ARI) in Beijing,five hundred and fifty-nine nasopharyngeal swab samples were collected from adult patients with ARI in Beijing. The prevalence of HCoV-OC43 infection among these patients was assessed using two different OneStep reverse transcription polymerase chain reaction (RT-PCR) assays. The epidemiological profiles of the patients… Expand
Molecular epidemiology and evolutionary histories of human coronavirus OC43 and HKU1 among patients with upper respiratory tract infections in Kuala Lumpur, Malaysia
The present study reported the molecular complexity and evolutionary dynamics of human betacoronaviruses among adults with acute respiratory symptoms in a tropical country and identified two novel HCoV-OC43 genetic lineages. Expand
Identification and evolutionary dynamics of two novel human coronavirus OC43 genotypes associated with acute respiratory infections: phylogenetic, spatiotemporal and transmission network analyses
The transmission network construction based on the TN93 pairwise genetic distance revealed the emergence and persistence of multiple sub-epidemic clusters of the highly prevalent genotype D and its descendant genotypes F and G, which contributed to the spread of HCoV-OC43 in the region. Expand
Genomic Analysis of 15 Human Coronaviruses OC43 (HCoV-OC43s) Circulating in France from 2001 to 2013 Reveals a High Intra-Specific Diversity with New Recombinant Genotypes
Identification of genetically distinct genotypes and eventually recombinant genotypes in samples collected in Lower Normandy between 2001 and 2013 provides important information for understanding the dynamics of the evolution of these epidemic coronaviruses. Expand
A six-year descriptive epidemiological study of human coronavirus infections in hospitalized patients in Hong Kong
A six-year epidemiological study on human coronavirus circulating in Hong Kong, using 8275 nasopharyngeal samples from patients with acute respiratory tract infections provides new insight into the epidemiology of coronaviruses. Expand
Safe and Sensitive Antiviral Screening Platform Based on Recombinant Human Coronavirus OC43 Expressing the Luciferase Reporter Gene
ABSTRACT Human coronaviruses (HCoVs) cause 15 to 30% of mild upper respiratory tract infections. However, no specific antiviral drugs are available to prevent or treat HCoV infections to date. Here,Expand
Innate Immune Antagonism by Diverse Coronavirus Phosphodiesterases
It is shown that MERS-CoV NS4b is a unique PDE with additional functions inhibiting the IFN response, a role dependent on both nuclear localization and its catalytic activity and the lab has studied coronavirus interactions with antiviral pathways activated by the presence of cytoplasmic viral double-stranded RNA (dsRNA). Expand
Prolonged Shedding of Human Coronavirus in Hematopoietic Cell Transplant Recipients: Risk Factors and Viral Genome Evolution
Summary Prolonged shedding of human coronavirus in hematopoietic cell transplant recipients was associated with initial high viral load, high-dose steroids, and myeloablative conditioning regimens.Expand


Molecular Epidemiology of Human Coronavirus OC43 Reveals Evolution of Different Genotypes over Time and Recent Emergence of a Novel Genotype due to Natural Recombination
This represents the first study to describe natural recombination in HCoV-OC43 and the evolution of different genotypes over time, leading to the emergence of novel genotype D, which is associated with pneumonia in the authors' elderly population. Expand
Prevalence of human coronaviruses in adults with acute respiratory tract infections in Beijing, China†
The demographic and clinical data present in this study of HCoV infections in adults with acute respiratory tract infections should improve the understanding of the pathogenesis of H coV infections. Expand
Characterization of Human Coronavirus Etiology in Chinese Adults with Acute Upper Respiratory Tract Infection by Real-Time RT-PCR Assays
All 4 non-SARS-associated HCoVs were more frequently detected by real-time RT-PCR assay in adults with URTI in Beijing and HCoV-229E led to the most prevalent infection. Expand
Severity and Outcome Associated With Human Coronavirus OC43 Infections Among Children
Background: Human coronaviruses are known causes of the common cold. Subtype OC43 (HCoV-OC43) is the more prevalent human coronavirus in several parts of the world. Recent studies have suggestedExpand
Genetic variability of human coronavirus OC43‐, 229E‐, and NL63‐like strains and their association with lower respiratory tract infections of hospitalized infants and immunocompromised patients
The detection of still untypeable hCoV strains suggests that the number of hCoVs involved in human pathology might further increase, and the use of multiple primer sets targeting different genes is recommended for diagnosis of all types of h coV infection. Expand
Epidemiology and Clinical Presentations of the Four Human Coronaviruses 229E, HKU1, NL63, and OC43 Detected over 3 Years Using a Novel Multiplex Real-Time PCR Method
The novel multiplex assay for real-time pan-coronavirus detection enhances respiratory virus diagnosis, overcomes potential diagnostic problems arising through seasonal variation in coronavirus frequency, and provides novel insights into the epidemiology and clinical implications of coronaviruses. Expand
An Outbreak of Coronavirus OC43 Respiratory Infection in Normandy, France
This study shows that an outbreak of HCoV OC43 respiratory infection was responsible for the lower respiratory tract symptoms observed in nearly one-third of patients identified by active surveillance for coronavirus infection. Expand
Direct diagnosis of human respiratory coronaviruses 229E and OC43 by the polymerase chain reaction
The RT-PCR-hybridization developed that amplified genetic material from the M protein gene of H CoV-229E and HCoV-OC43 could provide a useful tool for demonstrating the role of human coronavirus in infections of the respiratory tract. Expand
Inter- and intra-variant genetic heterogeneity of human coronavirus OC43 strains in France.
The presence of HCoV-OC43 as a quasispecies cloud in vivo during an acute respiratory-tract illness was discovered and it has been revealed that quasipecies-cloud sizes are similar for the two viral populations tested. Expand
Human coronaviruses are uncommon in patients with gastrointestinal illness
Investigation of the frequency of coronaviruses in stool samples from children and adults with gastrointestinal illness by RT-PCR identified HCoV-HKU1, a human coronavirus mainly associated with respiratory tract disease, as well as most individuals having respiratory findings as well. Expand