Presynaptic Activation of Silent Synapses and Growth of New Synapses Contribute to Intermediate and Long-Term Facilitation in Aplysia

Abstract

The time course and functional significance of the structural changes associated with long-term facilitation of Aplysia sensory to motor neuron synaptic connections in culture were examined by time-lapse confocal imaging of individual sensory neuron varicosities labeled with three different fluorescent markers: the whole-cell marker Alexa-594 and two presynaptic marker proteins-synaptophysin-eGFP to monitor changes in synaptic vesicle distribution and synapto-PHluorin to monitor active transmitter release sites. Repeated pulses of serotonin induce two temporally, morphologically, and molecularly distinct presynaptic changes: (1) a rapid activation of silent presynaptic terminals by filling of preexisting empty varicosities with synaptic vesicles, which parallels intermediate-term facilitation, is completed within 3-6 hr and requires translation but not transcription and (2) a slower generation of new functional varicosities which occurs between 12-18 hr and requires transcription and translation. Enrichment of empty varicosities with synaptophysin accounts for 32% of the newly activated synapses at 24 hr, whereas newly formed varicosities account for 68%.

DOI: 10.1016/S0896-6273(03)00595-6

Extracted Key Phrases

6 Figures and Tables

0204060'04'06'08'10'12'14'16
Citations per Year

278 Citations

Semantic Scholar estimates that this publication has 278 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Kim2003PresynapticAO, title={Presynaptic Activation of Silent Synapses and Growth of New Synapses Contribute to Intermediate and Long-Term Facilitation in Aplysia}, author={Joung-Hun Kim and Hiroshi Udo and Hsiu-Ling Li and Trisha Y Youn and Mary Chen and Eric R Kandel and Craig H Bailey}, journal={Neuron}, year={2003}, volume={40}, pages={151-165} }