Presteady-State and Steady-State Kinetics and Turnover Rate of the Mouse g-Aminobutyric Acid Transporter (mGAT3)

@article{Sacher2002PresteadyStateAS,
  title={Presteady-State and Steady-State Kinetics and Turnover Rate of the Mouse g-Aminobutyric Acid Transporter (mGAT3)},
  author={Ayelet Sacher and Nathan Alfred Nelson and Julie Ogi and Ernest M Wright and D S Loo and Sepehr Eskandari},
  journal={The Journal of Membrane Biology},
  year={2002},
  volume={190},
  pages={57-73}
}
We expressed mouse g-aminobutyric acid (GABA) transporter (mGAT3) in Xenopus laevis oocytes and examined its steady-state and presteady-state kinetics and turnover rate by using tracer flux and electrophysiological methods. In oocytes expressing mGAT3, GABA evoked a Na+-dependent and Cl?-facilitated inward current. The dependence on Na+ was absolute, whereas that for Cl? was not. At a membrane potential of ?50 mV, the half-maximal concentrations for Na+, Cl?, and GABA were 14 mM, 5 mM, and 3 mM… CONTINUE READING

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