Pressure effect on the kinetic of fluorescence photobleaching.

Abstract

We investigate by fluorescence microscopy the bulk fluorescence photobleaching of coumarin 1 and fluorescein in different solvents as a function of pressure up to 3.5 kbar. We show that for coumarin 1, the decrease in the fluorescence intensity is well described by a single exponential function whose characteristic time decreases with pressure following a… (More)
DOI: 10.1021/jp9119759

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