Preserving the structure of adsorbed protein films for time-of-flight secondary ion mass spectrometry analysis.

@article{Xia2003PreservingTS,
  title={Preserving the structure of adsorbed protein films for time-of-flight secondary ion mass spectrometry analysis.},
  author={Nan Nan Xia and David G. Castner},
  journal={Journal of biomedical materials research. Part A},
  year={2003},
  volume={67 1},
  pages={179-90}
}
The characterization of adsorbed protein films with ultrahigh vacuum (UHV) surface analysis techniques requires dehydration of the samples, which can cause significant alterations in protein structure. It is desirable to preserve the structure of adsorbed protein films during drying, so UHV analysis could be done in a state that is more representative of proteins' actual structure in the aqueous environment. In this study, two methods, trehalose protection and glutaraldehyde fixation, were… CONTINUE READING