Preserved and unpreserved 12 anti-allergic ophthalmic solutions and ocular surface toxicity: in vitro assessment in four cultured corneal and conjunctival epithelial cell lines.

@article{Ayaki2010PreservedAU,
  title={Preserved and unpreserved 12 anti-allergic ophthalmic solutions and ocular surface toxicity: in vitro assessment in four cultured corneal and conjunctival epithelial cell lines.},
  author={Masahiko Ayaki and Atsuo Iwasawa and Shigeo Yaguchi and Ryohei Koide},
  journal={Biocontrol science},
  year={2010},
  volume={15 4},
  pages={
          143-8
        }
}
In the present study, we evaluated the cytotoxicity of anti-allergic ophthalmic solutions in cultured corneal and conjunctival cells, namely SIRC (rabbit corneal epithelium), BCE C/D-1b (bovine corneal epithelial cells), RC-1 (rabbit corneal epithelium), and Chang (human conjunctival cells). The viability of cell cultures was determined following the exposure of cells to 12 commercially available anti-allergic ophthalmic solutions for varying exposure times and at various dilutions using the… 
In vitro assessment of the cytotoxicity of anti-allergic eye drops using 5 cultured corneal and conjunctival cell lines.
TLDR
Based on the penetration of the drug into eye tissues, HCECs are only likely to be pharmaceutically damaging in rare cases, and cell viability was found to be concentration-dependent.
In vitro effects of preserved and unpreserved anti-allergic drugs on human corneal epithelial cells.
TLDR
Stratified cell cultures seem to be a more relevant model for toxicity evaluation induced on the ocular surface epithelia than monolayer cultures and the presence of the preservative BAC in anti-allergic eye drop formulations contributes importantly to the cytotoxic effects induced by these compounds.
Toxicity of antiglaucoma drugs with and without benzalkonium chloride to cultured human corneal endothelial cells
TLDR
Assessment of the combined effect of drug and BAK revealed that latanoprost reduced the toxicity of BAK, and dilution of the antiglaucoma solutions resulted in markedly lower HCEC toxicity, HCEC damage due to antiglAUcoma medication may occur only in rare cases.
Multiple endpoint analysis of BAC-preserved and unpreserved antiallergic eye drops on a 3D-reconstituted corneal epithelial model
TLDR
BAC-preserved solutions decreased cell viability and induced apoptosis in a concentration-dependent manner and should be the first choice when treating allergic conjunctivitis, suggesting a better tolerance for the ocular surface.
Comparison of cytotoxicities and anti-allergic effects of topical ocular dual-action anti-allergic agents
TLDR
Alcaftadine seems to have less side effects and better therapeutic effects than the other two anti-allergic agents tested and may be more beneficial to use less toxic agents for patients with ocular surface risk factors or presumed symptoms of toxicity.
Cytotoxicity assays of new artificial tears containing 2-methacryloyloxyethyl phosphorylcholine polymer for ocular surface cells
TLDR
The MPC eye drops were tolerable to ocular surface cells, and comparable to single doses of clinically approved drugs containing sodium hyaluronate, although some significant differences were found.
Nano and microtechnologies for ophthalmic administration, an overview
TLDR
This review describes the main pharmaceutical nano- and microsystems currently under study to administer drugs to the eye, covering microparticles, nanoparticles, liposomes, microemulsions, niosomes and dendrimers.
Topical Clear Aqueous Nanomicellar Formulations For Anterior And Posterior Ocular Drug Delivery
TLDR
Stable nanomicellar constructs efficiently utilize their hydrophilic corona and evade the wash-out into the systemic circulation from both the conjunctival and choroidal blood vessels and lymphatics, and showed that NMFs were tolerable and delivered high drug concentrations to back-of-the-eye tissues with topical eye drop application to rabbits.
Ocular surface damage by ophthalmic compounds.
TLDR
Topical therapies may induce ocular surface allergic reactions, dry eye-like reactions, and epithelial damage, and patients in need of chronic therapies are at higher risk of facing these detrimental effects of eye drop therapies and should be treated with unpreserved compounds.
Ocular drug delivery systems: An overview.
TLDR
Current review intends to summarize the existing conventional formulations for ocular delivery and their advancements followed by current nanotechnology based formulation developments.
...
1
2
...

References

SHOWING 1-10 OF 11 REFERENCES
Comparative study of topical anti-allergic eye drops on human conjunctiva-derived cells: responses to histamine and IFNγ and toxicological profiles
BackgroundThe purpose of the study was to compare toxic effects and responses to histamine and IFNγ associated with the use of some widely used anti-allergic eye drops commercially available
Preservatives in eyedrops: The good, the bad and the ugly
TLDR
Care should be taken to avoid the long-term use of preservatives, otherwise a less toxic alternative to BAK should be developed, as this weakly allergenic but highly toxic compound exerts dose- and time-dependent effects.
Comparison of the conjunctival toxicity of topical ocular antiallergic agents.
  • Jong Soo Lee, J. E. Lee, Nami Kim, B. Oum
  • Medicine
    Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics
  • 2008
TLDR
When antiallergic agents are used to treat allergic conjunctivitis other than olopatadine, a particularly toxic effect on conjunctival cells associated with azelastine and ketotifen, rather than olopes, should be considered clinically.
Breakdown evaluation of corneal epithelial barrier caused by antiallergic eyedrops using an electrophysiologic method.
  • M. Nakashima, Tadahiro Nakamura, +7 authors H. Sasaki
  • Medicine
    Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics
  • 2008
TLDR
Monitoring changes in the corneal TEER, according to the electrophysiologic method with the application of antiallergic eyedrops, is useful for predictingCorneal epithelial breakdown caused by their instillation.
The effects of ophthalmic drugs, vehicles, and preservatives on corneal epithelium: a scanning electron microscope study.
TLDR
These studies show that the cytotoxicity of topical ocular preparations can be tested in an in vivo model and evaluated by scanning electron microscopy.
Tranilast inhibits cell proliferation and collagen synthesis by rabbit corneal and Tenon’s capsule fibroblasts
Purpose. To determine whether tranilast, N-(3,4-dimethoxycinnamoyl) anthranilic acid, influences cell proliferation and collagen synthesis by rabbit Tenon’s capsule fibroblasts (TFs) and corneal
Effect of Tranilast on Proliferation, Collagen Gel Contraction, and Transforming Growth Factor Beta Secretion of Retinal Pigment Epithelial Cells and Fibroblasts
TLDR
It is suggested that tranilast may inhibit the proliferation of RPE cells and fibroblasts and contraction of intraocular fibrous membranes by suppressing TGF-β1 secretion from these cells with a potential to treat PVR.
Electrophysiologic and morphologic effects of ophthalmic preparations on rabbit cornea epithelium.
TLDR
It was concluded that low concentrations of preservatives in ophthalmic preparations disrupt the barrier and transport properties of the corneal epithelium.
Effects of topical tranilast on corneal haze after photorefractive keratectomy
TLDR
Topical tranilast could reduce corneal haze by suppressing TGF‐β1 expression in keratocytes after photorefractive keratectomy, which showed significant difference compared with that of the control group.
Ibudilast in relapsing-remitting multiple sclerosis
TLDR
Preliminary evidence suggests that ibudilast seems to act in a neuroprotective fashion as measured by 2 independent MRI outcomes, with a possible beneficial clinical effect on disability progression.
...
1
2
...