Preservation of spatial organization and antigenicity of leukocyte surface molecules by aldehyde fixation: flow cytometry and high-resolution FESEM studies of CD62L, CD11b, and Thy-1.

@article{Hasslen1996PreservationOS,
  title={Preservation of spatial organization and antigenicity of leukocyte surface molecules by aldehyde fixation: flow cytometry and high-resolution FESEM studies of CD62L, CD11b, and Thy-1.},
  author={Sharon R. Hasslen and Alan R. Burns and Scott I Simon and Christopher W. J. Smith and Kathryn R. Starr and A. Neil Barclay and Sara A. Michie and R. David Nelson and Stanley L. Erlandsen},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={1996},
  volume={44 10},
  pages={1115-22}
}
We used transmission and scanning electron microscopy in conjunction with immunogold labeling to study cell surface molecules for evidence of distribution-function relationships. Ascription of functional significance to surface distribution therefore requires preservation of cell morphology and maintenance of molecular expression and distribution through the multiple steps of cell preparation. These requirements prompted us to compare two methods for preparing leukocytes for analysis of surface… CONTINUE READING