Preservation of immunorecognition by transferring cells from 10% neutral buffered formalin to 70% ethanol

@article{Otali2013PreservationOI,
  title={Preservation of immunorecognition by transferring cells from 10\% neutral buffered formalin to 70\% ethanol},
  author={Dennis Otali and Q. He and C R Stockard and W E Grizzle},
  journal={Biotechnic \& Histochemistry},
  year={2013},
  volume={88},
  pages={170 - 180}
}
Abstract Prolonged fixation of cells and tissues in 10% neutral buffered formalin (NBF) may decrease immunorecognition in some antigen-antibody pairs. Short fixation in 10% NBF followed by transfer to 70% ethanol has been used to overcome these effects, but the effects of this transfer on immunorecognition have not been explored adequately. We used two cell lines, DU145 (prostate cancer) and SKOV3 (ovarian cancer), grew them on coverslips and fixed them with 10% NBF at room temperature for 5… 
The Effect of Antigen Retrieval on Cells Fixed in 10% Neutral Buffered Formalin Followed by Transfer to 70% Ethanol
TLDR
It is indicated that 12 hrs of fixation in 10% NBF plus transfer to 70% ethanol for 4 weeks with AR resulted in recovery of immunorecognition for PCNA and cytokeratins, but standard methods of AR caused loss of Immunorec Cognition of EGFr.
Current Technologies for Fixation of Biological Material for Immunohistochemical Analysis (Review)
TLDR
None of the fixatives known to date has such a combination of properties that allow obtaining high-quality histological preparations and, at the same time, allows for detecting of any antigens in the stained tissue.
Usefulness of a tissue optical clearing technique for forensic autopsy
TLDR
Using this technique, tiny hemorrhages in the spinal cord were able to be identified by gross examination, allowing proper selection of locations for tissue sampling, and results obtained so far indicated that TOC could complement routine forensic autopsy procedures when detailed evaluation of small lesions is required.
Factors Affecting the Use of Human Tissues in Biomedical Research: Implications in the Design and Operation of a Biorepository.
TLDR
This review focuses on the limitations of the use of tissues in research and how the design and operations of a tissue biorepository can minimize some of these limitations.
Accomplishments of the Trustees and laboratory staff of the Biological Stain Commission, 2002–2013
  • R. Dapson
  • Biology
    Biotechnic & histochemistry : official publication of the Biological Stain Commission
  • 2014
Abstract During the 12 years from 2002 to 2013, the Trustees and laboratory personnel of the Biological Stain Commission (BSC) can claim many accomplishments. These accomplishments are itemized under
Scientific and Regulatory Policy Committee (SRPC) Review*
Increased cell proliferation is a central key event in the mode of action for many non-genotoxic carcinogens, and quantitative cell proliferation data play an important role in the cancer risk

References

SHOWING 1-10 OF 16 REFERENCES
Combined effects of formalin fixation and tissue processing on immunorecognition
TLDR
The results indicate that in addition to fixation, each of the tissue processing steps has effects on immunorecognition of the epitopes recognized by these antibodies, and suggests that AR should be used for staining PCNA.
Effects of isopropanol storage time on histochemical and immunohistochemical stains in lung tissue
TLDR
There were no significant differences in the staining intensities of hematoxylin and eosin, Masson’s trichrome, periodic acid Schiff, CD31, CD68, and smooth muscle actin in the lung tissue samples with increasing storage time in isopropanol, indicating that the tissues that are stored in isOPropanol at 4°C can yield good quality staining for research and clinical investigations.
Effects of fixation and tissue processing on immunohistochemical demonstration of specific antigens.
TLDR
It is found that in general neutral buffered formalin is the poorest fixative for maintaining antigen recognition by immunohistochemistry and that no single fixative was best for all antigens.
Macromolecular changes caused by formalin fixation and antigen retrieval
  • R. Dapson
  • Biology
    Biotechnic & histochemistry : official publication of the Biological Stain Commission
  • 2007
Although the mechanics of formalin fixation and antigen retrieval have been studied extensively and reviewed periodically, little attention has been directed toward conformational changes in target
Immunohistologic techniques for detecting the glycolipid Gb3 in the mouse kidney and nervous system
TLDR
The results of this study show that tissue preparation is crucial to the persistence and preservation of the glycolipid Gb3 in mouse tissue and may serve as a basis for determining the localization of other amphipathic Glycolipids in tissue.
Modeling formalin fixation and histological processing with ribonuclease A: effects of ethanol dehydration on reversal of formaldehyde cross-links
TLDR
A comparative evaluation of RNase A following fixation in buffered formaldehyde alone and after subsequent dehydration in 100% ethanol is performed by combining gel electrophoresis, chemical modification, and circular dichroism spectroscopic studies to reveal that ethanol-induced rearrangement of the conformation of fixedRNase A leads to protein aggregation through the formation of large geometrically compatible hydrophobic β-sheets that are likely stabilized by formaldehyde cross-links.
Special symposium: fixation and tissue processing models
  • W. Grizzle
  • Biology
    Biotechnic & histochemistry : official publication of the Biological Stain Commission
  • 2009
TLDR
Issues attending the use of 10% NBF combined with tissue processing as an interrelated process to study biomarkers identified by immunohistochemistry are reviewed.
Immunohistochemical detection of estrogen and progesterone receptor and HER2 expression in breast carcinomas: comparison of cell block and tissue block preparations.
TLDR
Compared ER, PR and HER2 expression in breast carcinomas using archival ethanol-fixed FNA cell block with formalin fixed resection tissue block preparations demonstrates the importance of adherence to the College of American Pathologists/ASCO guidelines for HER2 IHC.
Identification of Formaldehyde-induced Modifications in Proteins
TLDR
The use of model peptides enabled us to determine the reactivity of each particular cross-link reaction as a function of the reaction conditions and to identify new reaction products after incubation with formaldehyde.
...
...