Preservation of embryonic kidneys for transplantation.

  title={Preservation of embryonic kidneys for transplantation.},
  author={Martyn J Bottomley and Simona C Baicu and Janet M Boggs and Damien Marshall and Marc James Clancy and Kelvin G. M. Brockbank and Christopher A Bravery},
  journal={Transplantation proceedings},
  volume={37 1},
BACKGROUND Long-term storage of embryonic kidneys is crucial for the organization of transplantation and organ banking. In this study, we investigated the effects of controlled-rate freezing and ice-free vitrification on metanephroi (MN) viability. METHODS Metanephroi isolated from 15-day (E15) timed pregnant Lewis rats were either: (i) frozen, using a DMSO/FCS/RPMI solution and a controlled freezing rate of -0.3 degrees C/min, from -10 degrees to -40 degrees C; or (ii) cryopreserved in an… CONTINUE READING

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