Preservation of caprine preantral follicle viability after cryopreservation in sucrose and ethylene glycol

@article{Santos2006PreservationOC,
  title={Preservation of caprine preantral follicle viability after cryopreservation in sucrose and ethylene glycol},
  author={R R Santos and Theerawat Tharasanit and Jos{\'e} Ricardo de Figueiredo and Timon W. van Haeften and Robert van den Hurk},
  journal={Cell and Tissue Research},
  year={2006},
  volume={325},
  pages={523-531}
}
Caprine preantral follicles within ovarian fragments were cryopreserved in the absence or presence of 0.5 M sucrose with or without 1 M dimethyl sulfoxide and/or 1 M ethylene glycol (EG). After being thawed, they were washed in minimum essential medium with or without 0.3 M sucrose. Histological analysis of follicle integrity immediately after cryopreservation showed consistent beneficial effects of including sucrose in the three cryoprotectant solutions analyzed when tissue was thawed without… CONTINUE READING

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Cryopreservation and short-term culture of isolated caprine primordial follicles

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