Preservation of Thermophilic Bacterial Spores Using Filter Paper Disc Techniques

  title={Preservation of Thermophilic Bacterial Spores Using Filter Paper Disc Techniques},
  author={Kulkarni Ga and Ratnakar R. Chitte},
  journal={Journal of bioprocessing \& biotechniques},
Any microbial process requires a reliable source of culture stock that is reproducible, viable and taxonomically determined and also preserved without changes in genetic and phenotypic traits. Here we have described most effective and reproducible method for preservation of bacterial spores on filter disc at -20°C, 4°C and RT for use in laboratory for microbial process. The filter disc is used as base to support the spores inside the tiny holes. The filter paper discs are dipped into the medium… 

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