Preservation of Quercus robur germplasm by cryostorage of embryogenic cultures derived from mature trees and RAPD analysis of genetic stability.

@article{Snchez2008PreservationOQ,
  title={Preservation of Quercus robur germplasm by cryostorage of embryogenic cultures derived from mature trees and RAPD analysis of genetic stability.},
  author={Claudia S{\'a}nchez and M. T. Martinez and Nieves Vidal and M. Carmen San-Jos{\'e} and S Valladare and Ana Mar{\'i}a Vieitez},
  journal={Cryo letters},
  year={2008},
  volume={29 6},
  pages={493-504}
}
This study reports on the cryostorage of embryogenic lines derived from selected mature Quercus robur trees, following application of the PVS2-vitrification based procedure. In seven oak genotypes, embryo recovery levels ranging from 57-92% were obtained when 4-6 mg embryo clumps were precultured for 3 days on 0.3 M sucrose basal medium, treated with PVS2… CONTINUE READING