Preservation and detection of specific antibody—peptide complexes by matrix-assisted laser desorption ionization mass spectrometry

@article{Kiselar2000PreservationAD,
  title={Preservation and detection of specific antibody—peptide complexes by matrix-assisted laser desorption ionization mass spectrometry},
  author={Janna Kiselar and Kevin M. Downard},
  journal={Journal of the American Society for Mass Spectrometry},
  year={2000},
  volume={11},
  pages={746-750}
}
  • J. Kiselar, K. Downard
  • Published 2000
  • Chemistry, Medicine
  • Journal of the American Society for Mass Spectrometry
The direct detection of an antibody—peptide complex is reported by matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS). Experimental conditions have been found in which specific, noncovalent interactions in solution are maintained throughout the sample preparation and ionization process. Mass measurements based on the ion signals for the intact antibody and 1:1 antibody—peptide complex reveal that specific noncovalent associations between a monoclonal antibody and a peptide… Expand
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